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D1045 5-Hydroxymethyl dCTP

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  • 產(chǎn)品時間：2024-08-09
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D1045 5-Hydroxymethyl dCTP   

品牌：ZYMO Research

代理商：慧穎生物

庫存：貨期產(chǎn)品

D1045 5-Hydroxymethyl dCTP   

Ready to use 5-Hydroxymethylcytosine mix (dATP, dTTP, dGTP, d5hmCTP) and 5-Methylcytosine dNTP mix (dATP, dTTP, dGTP, d5mCTP) is of ultra high purity; >99% trisphosphate by HPLC.

Readily incorporated into PCR amplicons with ZymoTaq™ or other DNA polymerases.

Free of endo-, exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities.

About Hydroxy/Methylated Nucleotides

5-(Hydroxy)methylcytosine dNTP Mix and 5-(Hydroxy)methyl dCTP from Zymo Research are of ultra high purity and can be used to generate DNA by PCR using ZymoTaq™ or other DNA polymerases.

 For 5-hmCFor 5-mC
ReagentVolumeFinal ConcentrationVolumeFinal Concentration
2X Reaction Buffer25 µl1X25 µl1X
dNTP Mix0.5 µl0.25 mM each dNTP0.5 µl0.25 mM each dNTP
Forward Primer (10 µM)Variable0.3 to 1 µMVariable0.3 to 1 µM
Reverse Primer (10 µM)Variable0.3 to 1 µMVariable0.3 to 1 µM
Template DNAVariable< 200 ng/50 µlVariable< 200 ng/50 µl
ZymoTaq™ DNA Polymerase (5 U/µl)1 µl5 U/50 µl0.4 µl<2 U/50 µl
ddH20to 50 µl-to 50 µl-
Total Volume50 µl 50 µl 
 
Note: The final concentration of MgCl2 in the reaction (above) is 1.75 mM. If required, scale reaction reagent volumes accordingly to optimize the MgCl2, primer, and/or template concentrations.
Specifications
Concentration5-Hydroxymethylcytosine dNTP Mix: 2.5 µmol/250 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and d5hmCTP). 
5-Hydroxymethyl dCTP: 10µmol/100 µl as a [100 mM] solution.
5-Methylcytosine dNTP Mix: 2.5 µmol/250 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and d5mCTP).
5-Methyl dCTP: 1µmol/100 µl as a [10 mM] solution.
ApplicationsPCR amplicons containing 5-(hydroxy)methylcytosine are useful in a variety of applications such as: 
  • The study of 5-(hydroxy)methylcytosine binding/interacting protein
  • Define methods that distinguish between 5-methylcytosine and 5-hydroxymethylcytosine.
Storage5-(Hydroxy)methylcytosine dNTP Mix and 5-(Hydroxy)methyl dCTP are guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.

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